The recent outbreak of betacoronavirus Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which is responsible for the Coronavirus Disease 2019 (COVID-19) global pandemic, has created great challenges in viral diagnosis. The existing methods for nucleic acid detection are of high sensitivity and specificity, but the need for complex sample manipulation and expensive machinery slow down the disease detection. Thus, there is an urgent demand to develop a rapid, inexpensive, and sensitive diagnostic test to aid point-of-care viral detection for disease monitoring. In this study, we developed a clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated proteins (Cas) 12a-based diagnostic method that allows the results to be visualized by the naked eye. We also introduced a rapid sample processing method, and when combined with recombinase polymerase amplification (RPA), the sample to result can be achieved in 50 minutes with high sensitivity (1–10 copies per reaction). This accurate and portable detection method holds a great potential for COVID-19 control, especially in areas where specialized equipment is not available. The outbreak of SARS-CoV-2 has caused great damage to human society, and early detection of this virus is important to contain its spread and save lives. Existing diagnostic methods are complex and require well-trained personnel, but this study describes a fast and accurate method which can be operated by lay-users and whose results can be visualized by the naked eye.
【초록키워드】 COVID-19, SARS-CoV-2, equipment, diagnostic, Diagnosis, virus, coronavirus 2, Betacoronavirus, global pandemic, Spread, Protein, point-of-care, amplification, sensitivity, Nucleic acid detection, Sensitivity and specificity, outbreak, Viral detection, respiratory, disease, Diagnostic method, RPA, complex, polymerase, responsible, develop, caused, the disease, introduced, in viral, palindromic repeat, 【제목키워드】 SARS-CoV-2, Rapid,