Ecuador is one of the most affected countries, with the coronavirus disease 2019 (COVID-19) infection, in Latin America derived from an ongoing economic crisis. One of the most important methods for COVID-19 detection is the use of techniques such as real time RT-PCR based on a previous extraction/purification of RNA procedure from nasopharyngeal cells using functionalized magnetic nanoparticles (MNP). This technique allows the processing of ~ 10,000 tests per day in private companies and around hundreds per day at local Universities guaranteeing to reach a wide range of the population. However, the main drawback of this method is the need for specialized MNP with a strong negative charge for the viral RNA extraction to detect the existence of the SARS-CoV-2 virus. Here we present a simplified low cost method to produce 10 g of nanoparticles in 100 mL of solution that was scaled to one litter by parallelizing the process 10 times in just two days and allowing for the possibility of making ~ 50,000 COVID-19 tests. This communication helps in reducing the cost of acquiring MNP for diverse biomolecular applications supporting developing country budgets constraints and chemical availability specially during the COVID-19 International Health Emergency.
【저자키워드】 Nanoparticles, Synthesis and processing, 【초록키워드】 COVID-19, coronavirus disease, Infection, Local, RT-PCR, RNA, Latin America, Ecuador, university, Emergency, real time, help, COVID-19 tests, viral RNA extraction, negative charge, country, affected, detect, per day, reducing, MNP, nasopharyngeal cell, scaled, the SARS-CoV-2 virus, 【제목키워드】 SARS-CoV-2, RNA extraction, country,