The recurrence of new human cases of Middle East respiratory syndrome coronavirus (MERS-CoV) underscores the need for effective therapeutic countermeasures. Nonhuman primate models are considered the gold standard for preclinical evaluation of therapeutic countermeasures. However, MERS-CoV-induced severe respiratory disease in humans is associated with high viral loads in the lower respiratory tract, which may be difficult to achieve in nonhuman primate models. Considering this limitation, we wanted to ascertain the effectiveness of using a MERS-CoV infectious clone (icMERS-0) previously shown to replicate to higher titers than the wild-type EMC 2012 strain. We observed respiratory disease resulting from exposure to the icMERS-0 strain as measured by CT in rhesus monkeys with concomitant detection of virus antigen by immunohistochemistry. Overall, respiratory disease was mild and transient, resolving by day 30 post-infection. Although pulmonary disease was mild, these results demonstrate for the first time the utility of CT imaging to measure disease elicited by a MERS-CoV infectious clone system in nonhuman primate models.
【초록키워드】 immunohistochemistry, Human, virus, MERS-CoV, Antigen, Viral load, Respiratory disease, therapeutic, Effectiveness, Mild, utility, disease, pulmonary disease, Lower respiratory tract, Middle East, Post-infection, gold standard, severe respiratory disease, wild-type, respiratory syndrome coronavirus, clone, effective, Rhesus monkey, Infectious clone system, shown, resulting, replicate, elicited, 【제목키워드】 MERS-CoV, disease, rhesus macaque, Middle East, respiratory syndrome coronavirus, clone, elicit, mild respiratory,