Inactivation technology for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is certainly a critical measure to mitigate the spread of coronavirus disease 2019 (COVID-19). A deep ultraviolet light-emitting diode (DUV-LED) would be a promising candidate to inactivate SARS-CoV-2, based on the well-known antiviral effects of DUV on microorganisms and viruses. However, due to variations in the inactivation effects across different viruses, quantitative evaluations of the inactivation profile of SARS-CoV-2 by DUV-LED irradiation need to be performed. In the present study, we quantify the irradiation dose of DUV-LED necessary to inactivate SARS-CoV-2. For this purpose, we determined the culture media suitable for the irradiation of SARS-CoV-2 and optimized the irradiation apparatus using commercially available DUV-LEDs that operate at a center wavelength of 265, 280, or 300 nm. Under these conditions, we successfully analyzed the relationship between SARS-CoV-2 infectivity and the irradiation dose of the DUV-LEDs at each wavelength without irrelevant biological effects. In conclusion, total doses of 1.8 mJ/cm 2 for 265 nm, 3.0 mJ/cm 2 for 280 nm, and 23 mJ/cm 2 for 300 nm are required to inactivate 99.9% of SARS-CoV-2. Our results provide quantitative antiviral effects of DUV irradiation on SARS-CoV-2, serving as basic knowledge of inactivation technologies against SARS-CoV-2.
【저자키워드】 SARS-CoV-2, viral infection, Biophotonics, Inorganic LEDs, 【초록키워드】 COVID-19, coronavirus disease, viruses, coronavirus, knowledge, Variation, Antiviral effect, Spread, ultraviolet, inactivation, Quantitative, Critical, microorganism, dose, culture media, acute respiratory syndrome, Effect, mitigate, Effects, analyzed, performed, required, conditions, center wavelength, 【제목키워드】 ultraviolet, inactivation,