Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting classical restriction site (RS) sequence in human SARS-CoV-2 genomes and (2) comparing them with other recombinant SARS-CoV-like virus created for experimental purposes. Finally, we propose a novel approach consisting in the generation of a restriction endonucleases site map of SARS-CoV-2 and other related coronavirus genomes to be used as a fingerprint to trace the virus evolution.
All Keywords
【저자키워드】 viral infection, Molecular biology, genetic mapping, 【초록키워드】 Evolution, SARS-CoV-2, Genome, virus, endonuclease, China, Evidence, observation, sequence, human SARS-CoV-2, genetic manipulation, approach, classical, coronavirus genome, restriction site, 【제목키워드】 Evolution, SARS-CoV-2, endonuclease, Generation,
【저자키워드】 viral infection, Molecular biology, genetic mapping, 【초록키워드】 Evolution, SARS-CoV-2, Genome, virus, endonuclease, China, Evidence, observation, sequence, human SARS-CoV-2, genetic manipulation, approach, classical, coronavirus genome, restriction site, 【제목키워드】 Evolution, SARS-CoV-2, endonuclease, Generation,