Pseuodotyped particles have significant importance and use in virology as tools for studying the biology of highly pathogenic viruses in a lower biosafety environment. The biological, chemical, and serological studies of the recently emerged SARS-CoV-2 will be greatly aided by the development and optimization of a suitable pseudotyping system. Here, we pseudotyped the SARS-CoV-2 Spike glycoprotein (SPG) on a traditional retroviral (MMLV) as well as a third generation lentiviral (pLV) vector and tested the transduction efficiency in several mammalian cell lines expressing SARS-CoV-2 receptor hACE2. While MMLV pseudotyped the vesicular stomatitis virus G glycoprotein (VSV-G) efficiently, it could not pseudotype the full-length SPG. In contrast, pLV pseudotyped both glycoproteins efficiently; however, much higher titers of pLV-G particles were produced. Among all the tested mammalian cells, 293Ts expressing hACE2 were most efficiently transduced using the pLV-S system. The pLV-S particles were efficiently neutralized by diluted serum (>:640) from recently recovered COVID-19 patients who showed high SARS-CoV-2 specific IgM and IgG levels. In summary, pLV-S pseudotyped virus provides a valid screening tool for the presence of anti SARS-CoV-2 specific neutralizing antibodies in convalescent patient serum.
【저자키워드】 Microbiology, Virology, Preclinical research, 【초록키워드】 SARS-CoV-2, spike, virus, SARS-CoV-2 receptor, hACE2, Particle, biosafety, serum, Pseudotyped virus, pseudotype, glycoprotein, vesicular stomatitis virus, convalescent patient, Efficiency, IgG levels, 293T, specific IgM, mammalian cells, full-length, while, pseudotyped, highly pathogenic, G glycoprotein, mammalian cell, SEROLOGICAL STUDY, recovered COVID-19 patient, neutralized, produced, tested, provide, much higher, expressing, retroviral, transduced, lentiviral, specific neutralizing antibody, the SARS-CoV-2, VSV-G, 【제목키워드】 Particle, serum, SARS-CoV-2 spike glycoprotein, lentivirus, Patient, SARS-CoV-2 neutralizing antibody, pseudotyped,