The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing the limit of detection (LOD) is revealed for exceptionally large gold nanoparticles (AuNPs) with diameters of hundreds of nm. SPR enhanced by these large nanoparticles lowered the LOD of SARS-CoV-2 N protein to 85 fM, resulting in the highest SPR detection sensitivity ever obtained for SARS-CoV-2 N protein.
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【저자키워드】 Imaging and sensing, Optical materials and structures, 【초록키워드】 coronavirus, COVID-19 pandemic, SPR, Protein, limit of detection, N protein, SARS-CoV-2 N protein, Gold nanoparticle, Detection sensitivity, SARS-CoV-2 nucleocapsid, highest, resulting, significantly, the SARS-CoV-2, 【제목키워드】 Protein, SARS-CoV-2 nucleocapsid,
【저자키워드】 Imaging and sensing, Optical materials and structures, 【초록키워드】 coronavirus, COVID-19 pandemic, SPR, Protein, limit of detection, N protein, SARS-CoV-2 N protein, Gold nanoparticle, Detection sensitivity, SARS-CoV-2 nucleocapsid, highest, resulting, significantly, the SARS-CoV-2, 【제목키워드】 Protein, SARS-CoV-2 nucleocapsid,