Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt lum /Ct cyt ). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and host components and contribute to establish the basis to tackle the pathogenesis of SARS-CoV-2.
【저자키워드】 SARS-CoV-2, coronavirus, envelope protein, Topology, membrane insertion, 【초록키워드】 virus, E protein, membrane protein, membrane, Interaction, Sequence conservation, biochemical, similarities, component, C-terminus, mammalian cells, N-terminus, pathogenesis of SARS-CoV-2, Host, new SARS-CoV-2, defined, functional, contribute, cytoplasmic, translocated, 【제목키워드】 membrane, SARS-CoV-2 envelope, protein topology, eukaryotic,