COVID-19 displays diverse disease severities and symptoms including acute systemic inflammation and hypercytokinemia, with subsequent dysregulation of immune cells. Bacterial superinfections in COVID-19 can further complicate the disease course and are associated with increased mortality. However, there is limited understanding of how SARS-CoV-2 pathogenesis and hypercytokinemia impede the innate immune function against bacterial superinfections. We assessed the influence of COVID-19 plasma hypercytokinemia on the functional responses of myeloid immune cells upon bacterial challenges from acute-phase COVID-19 patients and their corresponding recovery-phase. We show that a severe hypercytokinemia status in COVID-19 patients correlates with the development of bacterial superinfections. Neutrophils and monocytes derived from COVID-19 patients in their acute-phase showed an impaired intracellular microbicidal capacity upon bacterial challenges. The impaired microbicidal capacity was reflected by abrogated MPO and reduced NETs production in neutrophils along with reduced ROS production in both neutrophils and monocytes. Moreover, we observed a distinct pattern of cell surface receptor expression on both neutrophils and monocytes, in line with suppressed autocrine and paracrine cytokine signaling. This phenotype was characterized by a high expression of CD66b, CXCR4 and low expression of CXCR1, CXCR2 and CD15 in neutrophils and low expression of HLA-DR, CD86 and high expression of CD163 and CD11b in monocytes. Furthermore, the impaired antibacterial effector function was mediated by synergistic effect of the cytokines TNF-α, IFN-γ and IL-4. COVID-19 patients receiving dexamethasone showed a significant reduction of overall inflammatory markers in the plasma as well as exhibited an enhanced immune response towards bacterial challenge ex vivo . Finally, broad anti-inflammatory treatment was associated with a reduction in CRP, IL-6 levels as well as length of ICU stay and ventilation-days in critically ill COVID-19 patients. Our data provides insights into the transient functional dysregulation of myeloid immune cells against subsequent bacterial infections in COVID-19 patients and describe a beneficial role for the use of dexamethasone in these patients. Author summary COVID-19 caused by SARS-CoV-2 induces immune-paralysis characterized by misdirected host responses and altered levels of inflammatory mediators. Additionally, bacterial superinfections can further exacerbate the disease. Here, we report an in-depth functional characterization of the effector response and phenotypic properties of neutrophils and monocytes derived from critically ill COVID-19 patients towards bacterial superinfection. We show that elevated levels of specific cytokine clusters, positively correlate with the development of bacterial superinfections in these patients. Neutrophils and monocytes of critically ill COVID-19 patients showed impaired bactericidal capacity, which was mediated by elevated inflammatory mediators in the plasma. The observed impaired bactericidal capacity in critically ill COVID-19 patients was due to reduced classical effector functions of neutrophils and monocytes. Interestingly, lower levels of overall inflammatory mediators as well as reduction in length of ICU stay and ventilation-days in critically ill COVID-19 patients treated with dexamethasone was observed. These data suggest that the exhaustion and paralysis of the cellular innate immunity against bacterial challenge in critically ill COVID-19 patients is driven by a misdirected host-response, characterized by a hyperinflammatory environment including dysregulated cytokine levels. These results further emphasize the utility and importance of dampening these inflammatory mediators via broad anti-inflammatory therapy in COVID-19 patients, which improves the antibacterial effector functions of neutrophils and monocytes.
【초록키워드】 COVID-19, Dexamethasone, Monocytes, SARS-CoV-2, immune response, Innate immunity, disease severity, neutrophil, hypercytokinemia, CRP, MPO, cytokine, Symptom, host response, bacterial superinfection, ICU, monocyte, Critically ill, response, Clusters, Bacterial infection, phenotype, immune cells, plasma, Inflammatory marker, systemic inflammation, utility, expression, SARS-CoV-2 pathogenesis, patients, HLA-DR, cytokine signaling, cytokine levels, function, COVID-19 patients, IFN-γ, TNF-α, Bacterial, IL-4, CD11b, cellular, Inflammatory mediators, Immune cell, CXCR2, COVID-19 patient, anti-inflammatory treatment, CXCR4, dysregulation, reduction, Ex vivo, Anti-inflammatory therapy, bacterial superinfections, low expression, increased mortality, host-response, phenotypic, CD163, CD66b, paralysis, ROS production, surface receptor, CXCR1, CD86, Hyperinflammatory, NET, Cell, synergistic, inflammatory mediator, IMPROVE, Course, classical, caused, subsequent, the disease, elevated, receiving, reduced, exhibited, characterized, treated, functional, provide, reflected, induce, suppressed, reduction in, dysregulated, driven by, abrogated, exacerbate, autocrine, complicate, IL-6 level, innate immune function, paracrine, 【제목키워드】 COVID-19, Bacterial, myeloid cell,