The immunogenicity of severe acute respiratory syndrome 2 virus (SARS-CoV-2) vaccines in immunocompromised patients remains to be further explored. Here, we evaluated the immunogenicity elicited by complete vaccination with BNT162b2 vaccine in solid organ transplant recipients (SOTRs). A cohort of 110 SOTRs from Northern Italy were vaccinated with two doses of BNT162b2 mRNA vaccine and prospectively monitored at baseline and after 42 days. Both SARS-CoV-2 naïve and recovered subjects were included. Humoral response elicited by vaccination, including SARS-CoV-2 neutralizing antibodies (SARS-CoV-2 NT Abs), was evaluated; additionally, ex-vivo ELISpot assay was performed for the quantification of Spike-specific T-cell response. Results were compared with those obtained in a cohort of healthy subjects. In a subset of patients, humoral and T-cell responses against delta variant were also evaluated. Less than 20% of transplanted subjects developed a positive humoral and cell-mediated response after complete vaccination schedule. Overall, median levels of immune response elicited by vaccination were significantly lower with respect to controls in SARS-CoV-2 naïve transplant, but not in SARS-CoV-2 recovered transplanted patients. Additionally, a significant impairment of both humoral and cell-mediated response was observed in mycophenolate-treated patients. Positive delta-SARS-CoV-2 NT Abs levels were detected in almost all the SARS-CoV-2 recovered subjects but not in previously uninfected patients. Our study supports previous observations of a low level of seroconversion after vaccination in transplanted patients.
【저자키워드】 SARS-CoV-2, BNT162b2 vaccine, immune response, Solid organ transplant recipients, 【초록키워드】 Vaccine, vaccination, mRNA vaccine, T-cell Response, variant, Italy, virus, Immunocompromised patient, Cohort, Seroconversion, Humoral response, Control, quantification, patients, SARS-CoV-2 neutralizing antibody, solid organ transplant, dose, humoral, cell-mediated response, Support, observation, acute respiratory syndrome, subject, healthy subjects, naïve, impairment, positive, recipient, significantly lower, uninfected, vaccination schedule, Complete, Result, evaluated, was performed, median, elicited, subset, baseline, ex-vivo ELISpot, of BNT162b2, the SARS-CoV-2, 【제목키워드】 Delta, BNT162b2, Transplant, response, Impact, recovered, mycophenolate, responsiveness, negative, Solid,