Background : The investigation of the antibody response to SARS-CoV-2 represents a key aspect in facing the COVID-19 pandemic. In the present study, we compared the new Immundiagnostik IDK® anti-SARS-CoV-2 S1 IgG assay with four widely-used commercial serological assays for the detection of antibodies targeting S (spike) and NC (nucleocapsid) proteins. Methods: Serum samples were taken from an unbiased group of convalescent patients and from a negative control group. Sample were simultaneously analyzed by the new Immundiagnostik IDK ® anti-SARS-CoV-2 S1 IgG assay, by the DiaSorin LIAISON ® SARS-CoV-2 S1/S2 IgG assay, and by the Euroimmun anti-SARS-CoV-2 S1 IgG ELISA. Antibodies binding NC were detected by the Abbott SARS-CoV-2 IgG assay and by the pan-immunoglobulin immunoassay Roche Elecsys ® anti-SARS-CoV-2. Moreover, we investigated samples of a group of COVID-19 convalescent subjects that were primarily tested S1 IgG non-reactive. Samples were also tested by live virus and pseudovirus neutralization tests. Results: Overall, the IDK® anti-SARS-CoV-2 S1 IgG assay showed the highest sensitivity among the evaluated spike (S) protein-based assays. Additionally, the Immundiagnostik assay correlated well with serum-neutralizing activity. Conclusions: The novel IDK ® anti-SARS-CoV-2 S1 IgG assay showed high sensitivity and specificity, representing a valid option for use in the routine diagnostic.
【저자키워드】 COVID-19, coronavirus, pandemic, antibody, neutralization, protection, sensitivity, Humoral response, titer, non-responder, 【초록키워드】 SARS-CoV-2, IgG, COVID-19 pandemic, diagnostic, Proteins, anti-SARS-CoV-2, ELISA, immunoassay, Serological assay, Sensitivity and specificity, pseudovirus, nucleocapsid, convalescent, Roche, Live virus, convalescent patient, binding, LIAISON, neutralization tests, subject, DiaSorin, S1/S2, serum sample, Abbott SARS-CoV-2 IgG, S1 IgG, anti-SARS-CoV-2 S1 IgG, negative control, highest, tested, analyzed, Sample, assays, investigated, evaluated, correlated, representing, the antibody response, Elecsy, 【제목키워드】 IgG, spike, detection, New,