BACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis that requires fast and coordinated responses. OBJECTIVES The objective of this work is to describe the isolation and propagation properties of SARS-CoV-2 isolates from the first confirmed cases of coronavirus disease 2019 (COVID-19) in Brazil. METHODS After diagnosis in patients that returned from Italy to the São Paulo city in late February by RT-PCR, SARS-CoV-2 isolates were obtained in cell cultures and characterised by full genome sequencing, electron microscopy and in vitro replication properties. FINDINGS The virus isolate was recovered from nasopharyngeal specimen, propagated in Vero cells (E6, CCL-81 and hSLAM), with clear cytopathic effects, and characterised by full genome sequencing, electron microscopy and in vitro replication properties. Virus stocks – viable (titre 2.11 × 10 6 TCID50/mL, titre 1.5 × 10 6 PFUs/mL) and inactivated from isolate SARS.CoV2/SP02.2020.HIAE.Br were prepared and set available to the public health authorities and the scientific community in Brazil and abroad. MAIN CONCLUSION We believe that the protocols for virus growth and studies here described and the distribution initiative may constitute a viable model for other developing countries, not only to help a rapid effective pandemic response, but also to facilitate and support basic scientific research.
【저자키워드】 COVID-19, SARS-CoV-2, virus isolation, Cell culture, virus network, 【초록키워드】 coronavirus disease, Brazil, coronavirus, pandemic, protocol, Diagnosis, in vitro, public health crisis, Italy, RT-PCR, Replication, Genome sequencing, virus isolate, Microscopy, Research, Patient, Isolation, Scientific community, distribution, inactivated, Support, confirmed case, acute respiratory syndrome, help, titre, cytopathic effects, nasopharyngeal specimen, Vero Cell, virus growth, responses, country, effective, resulting, described, facilitate, increase in, returned, public health authority, SARS-CoV-2 isolate, 【제목키워드】 Brazil, Patient, Isolation, reported,