Early detection of viral pathogens by DNA-sensors in clinical samples, contaminated foods, soil or water can dramatically improve clinical outcomes and reduce the socioeconomic impact of diseases such as COVID-19. Clustered regularly interspaced short palindromic repeat (CRISPR) and its associated protein Cas12a (previously known as CRISPR-Cpf1) technology is an innovative new-generation genomic engineering tool, also known as ‘genetic scissors’, that has demonstrated the accuracy and has recently been effectively applied as appropriate (E-CRISPR) DNA-sensor to detect the nucleic acid of interest. The CRISPR-Cas12a from Prevotella and Francisella 1 are guided by a short CRISPR RNA (gRNA). The unique simultaneous cis- and trans- DNA cleavage after target sequence recognition at the PAM site, sticky-end (5–7 bp) employment, and ssDNA/dsDNA hybrid cleavage strategies to manipulate the attractive nature of CRISPR–Cas12a are reviewed. DNA-sensors based on the CRISPR-Cas12a technology for rapid, robust, sensitive, inexpensive, and selective detection of virus DNA without additional sample purification, amplification, fluorescent-agent- and/or quencher-labeling are relevant and becoming increasingly important in industrial and medical applications. In addition, CRISPR-Cas12a system shows great potential in the field of E-CRISPR-based bioassay research technologies. Therefore, we are highlighting insights in this research direction. Graphical Abstract
【저자키워드】 COVID-19, SARS-CoV-2 virus, CRISPR-Cas, DNA-biosensors, CRISPR–Cas12a, Bioanalytical system, 【초록키워드】 virus, RNA, Protein, CRISPR, amplification, clinical samples, nucleic acid, DNA, Accuracy, Early detection, Research, cleavage, genomic, disease, Prevotella, employment, sequence, selective, viral pathogen, purification, gRNA, robust, detect, addition, applied, unique, demonstrated, reduce, increasingly, highlighting, Francisella, improve clinical outcome, PAM, 【제목키워드】 review, viral DNA,