Severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) are zoonotic pathogens with high fatality rates and pandemic potential. Vaccine development focuses on the principal target of the neutralizing humoral immune response, the spike (S) glycoprotein. Coronavirus S proteins are extensively glycosylated, encoding around 66–87 N-linked glycosylation sites per trimeric spike. Here, we reveal a specific area of high glycan density on MERS S that results in the formation of oligomannose-type glycan clusters, which were absent on SARS and HKU1 CoVs. We provide a comparison of the global glycan density of coronavirus spikes with other viral proteins including HIV-1 envelope, Lassa virus glycoprotein complex, and influenza hemagglutinin, where glycosylation plays a known role in shielding immunogenic epitopes. Overall, our data reveal how organisation of glycosylation across class I viral fusion proteins influence not only individual glycan compositions but also the immunological pressure across the protein surface. Glycosylation plays a key role in shielding of immunogenic epitopes on viral spike (S) proteins. Here Watanabe et al. report that glycans of coronavirus SARS and MERS S proteins are heterogeneously distributed and do not form an efficacious high-density global shield which would ensure efficient immune evasion.
【저자키워드】 glycobiology, Viral immune evasion, Glycoproteins, 【초록키워드】 coronavirus, pandemic, S protein, glycosylation, spike, Proteins, MERS, Protein, immune evasion, Epitopes, HIV-1, Clusters, Neutralizing, glycoprotein, humoral immune response, fusion protein, epitope, HKU1, glycan, Middle East, shielding, acute respiratory syndrome, Viral protein, complex, syndrome, Fatality rate, CoVs, immunogenic, viral spike, trimeric spike, high-density, zoonotic pathogen, immunological, N-linked glycosylation, coronavirus, glycosylated, influenza hemagglutinin, virus glycoprotein, 【제목키워드】 coronavirus, glycosylation, vulnerability,