Coronaviruses of bats and pangolins have been implicated in the origin and evolution of the pandemic SARS-CoV-2. We show that spikes from Guangdong Pangolin-CoVs, closely related to SARS-CoV-2, bind strongly to human and pangolin ACE2 receptors. We also report the cryo-EM structure of a Pangolin-CoV spike protein and show it adopts a fully-closed conformation and that, aside from the Receptor-Binding Domain, it resembles the spike of a bat coronavirus RaTG13 more than that of SARS-CoV-2. It has been suggested that pangolin coronaviruses may be the origin of SARS-CoV-2. Here the authors show that the Pangolin-CoV spike is structurally closely related to the closed form of SARS-CoV-2 spike and exhibits similar binding properties to human and pangolin ACE2; although neither spike binds bat ACE2.
【저자키워드】 SARS-CoV-2, Cryoelectron microscopy, 【초록키워드】 Evolution, coronavirus, pandemic, spike, Spike protein, RaTG13, ACE2 receptors, bat, binding, SARS-CoV-2 spike, pangolin, Guangdong, cryo-EM structure, bind, suggested, implicated, exhibit, bat ACE2, 【제목키워드】 Structure, spike glycoprotein, Evolution of SARS-CoV-2, binding,