Abstract The scientific community has responded to the coronavirus disease 2019 (COVID-19) pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease. Encouragingly, researchers from a diverse array of fields are collectively working towards this goal. Research with infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is undertaken in high-containment laboratories; however, it is often desirable to work with samples at lower-containment levels. To facilitate the transfer of infectious samples from high-containment laboratories, we have tested methods commonly used to inactivate virus and prepare the sample for additional experiments. Incubation at 80°C, a range of detergents, Trizol reagents, and UV energies were successful at inactivating a high titer of SARS-CoV-2. Methanol and paraformaldehyde incubation of infected cells also inactivated the virus. These protocols can provide a framework for in-house inactivation of SARS-CoV-2 in other laboratories, ensuring the safe use of samples in lower-containment levels. SARS-CoV-2 research requires the safe use of samples in low-containment laboratories. Heat, UV, and chemical reagents inactivated high titers of virus in solution and cells. These methods will facilitate use of once-infectious samples in downstream procedures.
【저자키워드】 SARS-CoV-2, inactivation, temperature, methanol, Trizol, detergents, paraformaldehyde, 【초록키워드】 COVID-19, coronavirus disease, coronavirus, pandemic, protocol, virus, cells, Research, Scientific community, Incubation, disease, inactivated, Safe, acute respiratory syndrome, reagents, SARS-CoV-2 research, transfer, heat, reagent, infected cell, downstream, researcher, tested, facilitate, reduce, experiments, effective strategy, infectious sample, 【제목키워드】 Biological,