A rapid, sensitive and simple point-of-care (POC) nucleic acid diagnostic test is needed to prevent spread of infectious diseases. Paper-based toehold reaction, a recently emerged colorimetric POC nucleic acid diagnostic test, has been widely used for pathogen detection and microbiome profiling. Here, we introduce an amplification method called reverse transcription loop-mediated amplification (RT-LAMP) prior to the toehold reaction and modify it to enable more sensitive and faster colorimetric detection of RNA viruses. We show that incorporating the modified RT-LAMP to the toehold reaction detects as few as 120 copies of coronavirus RNA in 70 min. Cross-reactivity test against other coronaviruses indicates this toehold reaction with the modified RT-LAMP is highly specific to the target RNA. Overall, the paper-based toehold switch sensors with the modified RT-LAMP allow fast, sensitive, specific and colorimetric coronavirus detection.
【저자키워드】 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), molecular diagnostics, Reverse transcription loop-mediated isothermal amplification (RT-LAMP), Middle East respiratory syndrome coronavirus (MERS-CoV), 【초록키워드】 coronavirus, Diseases, POC, diagnostic, RNA, Spread, point-of-care, amplification, colorimetric detection, nucleic acid, RT-LAMP, RNA viruses, Microbiome, reverse transcription, pathogen detection, coronavirus RNA, Prevent, detect, other coronavirus, indicate, faster, modify, 【제목키워드】 detection, RNA, sensor, switch,