Previous studies reported on the broad-spectrum antiviral function of heparin. Here we investigated the antiviral function of magnesium-modified heparin and found that modified heparin displayed a significantly enhanced antiviral function against human adenovirus (HAdV) in immortalized and primary cells. Nuclear magnetic resonance analyses revealed a conformational change of heparin when complexed with magnesium. To broadly explore this discovery, we tested the antiviral function of modified heparin against herpes simplex virus type 1 (HSV-1) and found that the replication of HSV-1 was even further decreased compared to aciclovir. Moreover, we investigated the antiviral effect against the new severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) and measured a 55-fold decreased viral load in the supernatant of infected cells associated with a 38-fold decrease in virus growth. The advantage of our modified heparin is an increased antiviral effect compared to regular heparin.
【저자키워드】 SARS-CoV-2, Antiviral, Adenovirus, HSV-1, NMR, modified heparin, magnesium chloride, 【초록키워드】 coronavirus, virus, Antiviral effect, Replication, Viral load, nuclear magnetic resonance, conformational change, primary cells, acute respiratory syndrome, previous study, infected cell, supernatant, herpes simplex, virus growth, decrease, tested, significantly, reported, investigated, analysis, immortalized, 【제목키워드】 heparin, Magnesium, Spectrum, function, enhanced,