Various pathogens, such as Ebola virus, Marburg virus, Nipah virus, Hendra virus, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle East Respiratory Syndrome Coronavirus (MERS-CoV), and SARS-CoV-2, are threatening human health worldwide. The natural hosts of these pathogens are thought to be bats. The rousette bat, a megabat, is thought to be a natural reservoir of filoviruses, including Ebola and Marburg viruses. Additionally, the rousette bat showed a transient infection in the experimental inoculation of SARS-CoV-2. In the current study, we established and characterized intestinal organoids from Leschenault’s rousette, Rousettus leschenaultii . The established organoids successfully recapitulated the characteristics of intestinal epithelial structure and morphology, and the appropriate supplements necessary for long-term stable culture were identified. The organoid showed susceptibility to Pteropine orthoreovirus (PRV) but not to SARS-CoV-2 in experimental inoculation. This is the first report of the establishment of an expandable organoid culture system of the rousette bat intestinal organoid and its sensitivity to bat-associated viruses, PRV and SARS-CoV-2. This organoid is a useful tool for the elucidation of tolerance mechanisms of the emerging rousette bat-associated viruses such as Ebola and Marburg virus.
【저자키워드】 SARS-CoV-2, ACE2, bat, Organoid, natural host, long-term stable culture, virus susceptibility, TMPRSS2, 【초록키워드】 viruses, SARS-CoV, susceptibility, Infection, virus, MERS-CoV, sensitivity, Health, Characteristics, Culture, pathogen, Pathogens, morphology, Ebola, Nipah virus, respiratory, epithelial, bats, Ebola virus, filoviruses, Middle East, Host, Hendra virus, intestinal, thought, characterized, Rousettus, intestinal organoid, tolerance mechanism, 【제목키워드】 Rousettus,