G-quadruplexes are the non-canonical nucleic acid structures that are preferentially formed in G-rich regions. This structure has been shown to be associated with many biological functions. Regardless of the broad efforts on DNA G-quadruplexes, we still have limited knowledge on RNA G-quadruplexes, especially in a transcriptome-wide manner. Herein, by integrating the DMS-seq and the bioinformatics pipeline, we profiled and depicted the RNA G-quadruplexes in the human transcriptome. The genes that contain RNA G-quadruplexes in their specific regions are significantly related to immune pathways and the COVID-19-related gene sets. Bioinformatics analysis reveals the potential regulatory functions of G-quadruplexes on miRNA targeting at the scale of the whole transcriptome. In addition, the G-quadruplexes are depleted in the putative, not the real, PAS-strong poly(A) sites, which may weaken the possibility of such sites being the real cleaved sites. In brief, our study provides insight into the potential function of RNA G-quadruplexes in post-transcription.
【저자키워드】 Transcriptome, G-quadruplex, post-transcriptional regulation, miRNA targeting, RG4, RNA G-quadruplex, 【초록키워드】 Structure, Biological functions, knowledge, bioinformatics, miRNA, RNA, Regulatory, nucleic acid, DNA, Region, function, Analysis, effort, human transcriptome, regions, shown, significantly, addition, provide, reveal, cleaved, immune pathway, transcriptome-wide, 【제목키워드】 Human, RNA, Profiling, endogenous,