Genotyping single-nucleotide polymorphisms (SNPs) in microbiomes enables strain-level quantification. In this protocol, we describe a computational pipeline that performs fast and accurate SNP genotyping using metagenomic data. We first demonstrate how to use Maast to catalog SNPs from microbial genomes. Then we use GT-Pro to extract unique SNP-covering k-mers, optimize a data structure for storing these k-mers, and finally perform metagenotyping. For proof of concept, the protocol leverages public whole-genome sequences to metagenotype a synthetic community.
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【저자키워드】 Microbiology, bioinformatics, Genomics, genetics, Evolutionary biology, Sequence analysis,
【저자키워드】 Microbiology, bioinformatics, Genomics, genetics, Evolutionary biology, Sequence analysis,