A: Schematic of SCENIC workflow. Transcription factor/cofactor activity was predicted based on co-expression modules between factors and candidate target genes and calculated as AUC scores. TF, transcription factor. B: Heatmap showing average regulon activity of hepatocytes for each condition. Representative enriched GO terms related to corresponding regulons are indicated on the right side. C: UMAP visualization showing regulon activity of EZH2 (top) and PPARG (bottom) for indicated conditions. Red circles indicate cycling hepatocyte cluster (upper) and pericentral/midzonal/periportal hepatocyte clusters (bottom). D: GRNs in hepatocytes visualized by Cytoscape. Selected target genes of each regulon are labeled in the network. E: Heatmap showing average regulon activity of HSCs for each condition. Representative enriched GO terms related to the corresponding regulons are indicated on the right side. F: UMAP visualization showing regulon activity of TEAD1 for the indicated conditions. Red circles indicate HSC cluster. G: GRNs in HSCs visualized by Cytoscape. Selected target genes for each regulon are labeled in the network.
【저자키워드】 Toxicity, Angiogenesis, Gene regulatory networks, Hepatic stellate cells, Liver fibrosis, hepatocytes, cell-cell interactions, Single-cell and single-nucleus RNA-sequencing,