Duck hepatitis A virus 1 ( DHAV-1 ) is the primary cause of duck viral hepatitis, leading to sudden mortality in ducklings and significant economic losses in the duck industry. However, little is known about how DHAV-1 affects duckling liver at the molecular level. We conducted an analysis comparing the expression patterns of mRNAs and miRNAs in DHAV-1-infected duckling livers to understand the underlying mechanisms and dynamic changes. We identified 6,818 differentially expressed mRNAs ( DEGs ) and 144 differentially expressed microRNAs ( DEMs ) during DHAV-1 infection. Functional enrichment analysis of DEGs and miRNA target genes using gene ontology ( GO ) and Kyoto Encyclopedia of Genes and Genomes ( KEGG ) revealed their potential involvement in innate antiviral immunity, mitophagy, and pyroptosis. We constructed coexpression networks of mRNA-miRNA interactions and confirmed key DEMs (novel-mir333, novel-mir288, novel-mir197, and novel-mir71) using RT-qPCR. Further investigation demonstrated that DHAV-1 activates the RLRs signaling pathway, disrupts mitophagy, and induces pyroptosis. In conclusion, DHAV-1-induced antiviral immunity is closely linked to mitophagy, suggesting it could be a promising therapeutic target.
【저자키워드】 liver, mitophagy, DHAV-1, RLRs signaling pathway,