Context: Maternal exercise improves infant mesenchymal stem cell (MSC) fatty acid metabolism and insulin sensitivity; however, it remains unknown if these effects are associated with changes in MSC mitochondrial content and capacity. Objective: To elucidate the effects of different modes of maternal exercise on offspring umbilical cord-derived MSC mitochondrial capacity and content. Design: Randomized controlled trial. Setting: Clinical research facility. Patients: Pregnant females (n=43) between 18 and 39 years of age and <16 weeks’ gestation. Participants were free from chronic conditions and were not taking medications that may affect fetal development. Intervention: Women were randomized to either 150 minutes of moderate-intensity aerobic (AE, n=10), resistance (RE, n=12), or combination (CE, n=9) exercise per week, or to a non-exercising control (CTRL, n=12). Main Outcome Measures: At delivery, MSCs were isolated from the patients’ umbilical cords. MSC glucose and fatty metabolism were assessed using radiolabeled substrates. Mitochondrial capacity was assessed using high-resolution respirometry. Results: MSCs from the offspring of all the exercising women display lower mitochondrial state 3 and state 3u respiration (p<0.01), without any differences in mitochondrial content (p>0.05). Mitochondrial state 3 respiration is negatively associated with MSC mitochondrial efficiency for fatty acid oxidation, but not MSC insulin action. Lower mitochondrial capacity was associated with lower infant birthweight and adiposity at 1 month of age. Conclusion: Our data demonstrate that maternal exercise, irrespective of exercise mode, decreases mitochondrial state 3 and state 3u respiration without affecting MSC mitochondrial content.
【저자키워드】 Mitochondria, exercise, Pregnancy, Pediatric Obesity, Fat metabolism,