Specialized T cells are located in the nasal cavity and act as the first line of defense against respiratory viral infection. Here, we present a protocol for the detection and characterization of antigen-specific nasal-resident T cells. We detail steps for localized nasal swabbing to collect the nasal samples. We then describe IFN-γ ELISpot and an activation-induced marker assay to detect and characterize antigen-specific nasal-resident T cells.
All Keywords
【저자키워드】 immunology, Microbiology, Cell isolation, Cell-based Assays, Health Sciences, clinical protocol,
【저자키워드】 immunology, Microbiology, Cell isolation, Cell-based Assays, Health Sciences, clinical protocol,