Background Mesenchymal stem cells derived from human umbilical cord (hUC-MSCs) have immunomodulatory properties that are of interest to treat novel coronavirus disease 2019 (COVID-19). Leng et al. recently reported that hUC-MSCs derived from one donor negatively expressed Angiotensin-Converting Enzyme 2 (ACE2), a key protein for viral infection along with Transmembrane Serine Protease 2 (TMPRSS2). The purpose of this study was to quantify the expression of ACE2 and TMPRSS2 in hUC-MSCs lots derived from multiple donors using molecular-based techniques in order to demonstrate their inability to be a host to SARS-CoV-2. Methods Expression of ACE2 and TMPRSS2 was analyzed in 24 lots of hUC-MSCs derived from Wharton’s jelly via quantitative polymerase chain reaction (qPCR), Western Blot, immunofluorescence and flow cytometry using 24 different donors. Results hUC-MSCs had significantly lower ACE2 (p = 0.002) and TMPRSS2 (p = 0.008) expression compared with human lung tissue homogenates in Western blot analyses. Little to no expression of ACE2 was observed in hUC-MSC by qPCR, and they were not observable with immunofluorescence in hUC-MSCs cell membranes. A negative ACE2 and TMPRSS2 population percentage of 95.3% ± 15.55 was obtained for hUC-MSCs via flow cytometry, with only 4.6% ACE2 and 29.5% TMPRSS2 observable positive populations. Conclusions We have demonstrated negative expression of ACE2 and low expression of TMPRSS2 in 24 lots of hUC-MSCs. This has crucial implications for the design of future therapeutic options for COVID-19, since hUC-MSCs would have the ability to “dodge” viral infection to exert their immunomodulatory effects. Supplementary Information The online version contains supplementary material available at 10.1186/s12967-021-02813-6.
【저자키워드】 COVID-19, SARS-CoV2, Mesenchymal stem cells, ACE2 expression, TMPRSS2 expression, HUC-MSC, TMPRSS2, 【초록키워드】 SARS-CoV-2, viral infection, ACE2, immunomodulatory effects, novel coronavirus disease, flow cytometry, Protein, qPCR, donors, immunofluorescence, expression, Quantitative, mesenchymal stem cell, Donor, supplementary material, low expression, treat, cell membranes, positive, human lung tissue, significantly lower, Host, implication, populations, polymerase chain, Blot, Result, analyzed, reported, expressed, demonstrated, analyses, was obtained, future therapeutic, immunomodulatory property, umbilical, 【제목키워드】 ACE2, stem cell, low expression, umbilical,