Three CK2β variants with incorporated para ‐azidophenylalanine at different positions were mixed each with DU145 cell lysate and photo‐crosslinking was performed by UV light irradiation. Cross‐linked products were enriched by NiNTA affinity chromatography, digested by trypsin and identified by LC–MS. In total, 43 different proteins coupled to CK2β were found. Binding of HSP70‐1 as one of these was confirmed by microscale thermophoresis and co‐immunoprecipitation experiments in three different cancer cell lines.
All Keywords
【저자키워드】 Prostate cancer, HSP70‐1, photo‐crosslinking mass spectrometry, protein kinase CK2, Rho guanin nucleotide exchange factor 12,
【저자키워드】 Prostate cancer, HSP70‐1, photo‐crosslinking mass spectrometry, protein kinase CK2, Rho guanin nucleotide exchange factor 12,