Miao et al. genetically encoded RBD and ACE2 with Halo/SNAP tags and then labeled them with rhodamine/cyanine dyes. SIM imaging resolved endocytosis of RBD-ACE2 binding in living cells, including RBD-ACE2 recognition on the plasma membrane, cofactor-regulated membrane internalization, RAB-bearing vesicle formation and transport, RAB degradation, and downregulation of ACE2.
All Keywords
【저자키워드】 COVID-19, SARS-COV-2 infection, endocytosis, super-resolution imaging, Halo tag, RBD degradation, RBD-ACE2 interaction, self-labeling, SIM imaging, SNAP tag,
【저자키워드】 COVID-19, SARS-COV-2 infection, endocytosis, super-resolution imaging, Halo tag, RBD degradation, RBD-ACE2 interaction, self-labeling, SIM imaging, SNAP tag,