Background: There is much data available concerning the initiation of the immune response after SARS-CoV-2 infection, but long-term data are scarce. Methods: We thus longitudinally evaluated and compared the total and neutralizing immune response of 61 patients to SARS-CoV-2 infection up to eight months after diagnosis by RT–PCR using several commercial assays. Results: Among the 208 samples tested, the percentage of seropositivity was comparable between assays up to four months after diagnosis and then tended to be more heterogeneous between assays ( p < 0.05). The percentage of patients with a neutralizing titer decreased from 82% before two months postdiagnosis to 57% after six months. This decrease appeared to be more marked for patients under 65 years old and those not requiring hospitalization. The percentage of serology reversion at 6 months was from 11% with the WANTAI total assay to over 39% with the ABBOTT IgG assay. The neutralizing antibody titers decreased in parallel with the decrease of total antibody titers, with important heterogeneity between assays. Conclusions: In conclusion, serological tests show equivalent sensitivity in the first months after the diagnosis of SARS-CoV-2 infection, but their performance later, postinfection, must be considered when interpreting the results.
【저자키워드】 COVID-19, SARS-CoV-2, serological assays, assay performance, 【초록키워드】 serological test, IgG, immune response, Hospitalization, serology, SARS-COV-2 infection, Infection, Diagnosis, heterogeneity, sensitivity, Patient, Neutralizing, Antibody titers, Neutralizing antibody titer, Neutralizing titer, heterogeneous, RT–PCR, decrease, Postinfection, tested, assays, evaluated, eight, comparable, concerning, diagnosis of SARS-CoV-2, 【제목키워드】 longitudinal, Month,