Background Coronavirus disease 2019 (COVID-19) has been shown to cause serious health problems among them is the Acute Respiratory Distress syndrome (ARDS). Vitamin D receptor (VDR) signaling possibly partakes in the pathophysiology of this devastating complication. Methods In the current project, we have appraised expression levels of VDR , CYP27B1 and a number of associated lncRNAs in the circulation of COVID-19 patients versus healthy subjects using real-time PCR method. Results Expression of SNHG6 was considerably lower in COVID-19 patients compared with control subjects (Ratio of mean expression (RME) = 0.22, P value = 7.04E-05) and in both female and male COVID-19 patients compared with sex-matched unaffected individuals (RME = 0.32, P value = 0.04 and RME = 0.16, P value = 0.000679683, respectively). However, its expression was similar among ICU-hospitalized and non-ICU patients. Similarly, expression of SNHG16 was lower in in COVID-19 patients compared with controls (RME = 0.20, P value = 5.94E-05) and in both female and male patients compared with sex-matched controls (RME = 0.32, P value = 0.04 and RME = 0.14, P value = 0.000496435, respectively) with no significant difference among ICU-hospitalized and non-ICU hospitalized patients. Expression of VDR was lower in COVID-19 patients compared with controls (RME = 0.42, P value = 0.04) and in male patients compared with male controls (RME = 0.27, P value = 0.02). Yet, expression of VDR was statistically similar between female subgroups and between ICU-hospitalized and non-ICU hospitalized patients. Expression levels CYP27B , Linc00511 and Linc00346 were similar among COVID-19 patients and healthy subjects or between their subgroups. Significant correlations have been detected between expression levels of VDR , CYP27B and SNHG6 , SNHG16 , Linc00511 and Linc00346 lncRNAs both among COVID-19 patients and among healthy controls with the most significant ones being SNHG6 and SNHG16 (r = 0.74, P value = 3.26e-17 and r = 0.81, P = 1.54e-22, respectively). Conclusion Combination of transcript levels of VDR , CYP27B and SNHG6 , SNHG16 , Linc00511 and Linc00346 could differentiate patients from controls with AUC = 0.76, sensitivity = 0.62 and specificity = 0.81. The current data potentiate SNHG6 , SNHG16 and VDR as possible contributors in COVID-19 infection but not in the severity of ARDS.
【저자키워드】 COVID-19, Vitamin D receptor, VDR, lncRNA, SNHG6, SNHG16, Linc00511, Linc00346, CYP27B, 【초록키워드】 coronavirus disease, Respiratory distress syndrome, Coronavirus disease 2019, ARDS, acute respiratory distress syndrome, Vitamin D, severity, hospitalized patients, Health, COVID-19 infection, Real-time PCR, pathophysiology, male, female, Patient, Control, Vitamin D receptor, SNHG6, SNHG16, Linc00511, receptor, circulation, respiratory, correlation, expression, COVID-19 patients, Signaling, COVID-19 patient, respiratory distress, health problems, p value, subgroup, control subjects, no significant difference, expression level, healthy control, healthy subjects, subgroups, individual, syndrome, non-ICU, problem, healthy controls, expression levels, non-ICU patients, CYP27B1, transcript levels, Result, shown, transcript level, healthy subject, Significant, Ratio, statistically, control subject, 【제목키워드】 expression, COVID-19 patient,