Detection of SARS-CoV-2 is routinely performed in naso/oropharyngeal swabs samples from patients via RT-qPCR. The RT-LAMP technology has also been used for viral RNA detection in respiratory specimens with both high sensitivity and specificity. Recently, we developed a novel RT-LAMP test for SARS-CoV-2 RNA detection in nasopharyngeal swab specimens (named, N15-RT-LAMP) that can be performed as a single-tube colorimetric method, in a real-time platform, and as dry-LAMP. To date, there has been very little success in detecting SARS-CoV-2 RNA in urine by RT-qPCR, and the information regarding urine viral excretion is still scarce and not comprehensive. Here, we tested our N15-RT-LAMP on the urine of 300 patients admitted to the Hospital of Salamanca, Spain with clinical suspicion of COVID-19, who had a nasopharyngeal swab RT-qPCR-positive ( n = 100), negative ( n = 100), and positive with disease recovery ( n = 100) result. The positive group was also tested by RT-qPCR for comparison to N15-RT-LAMP. Only a 4% positivity rate was found in the positive group via colorimetric N15-RT-LAMP and 2% via RT-qPCR. Our results are consistent with those obtained in other studies that the presence of SARS-CoV-2 RNA in urine is a very rare finding. The absence of SARS-CoV-2 RNA in urine in the recovered patients might suggest that the urinary route is very rarely used for viral particle clearance.
【저자키워드】 COVID-19, SARS-CoV-2, molecular diagnostics, RT-LAMP, RT-qPCR, Urine, 【초록키워드】 detection, Nasopharyngeal swab, Sensitivity and specificity, Patient, Spain, SARS-CoV-2 RNA, Viral RNA, Swab, information, disease, platform, SARS-CoV-2 RNA detection, clinical suspicion, positive, clearance, viral particle, nasopharyngeal swab specimen, Salamanca, recovered patient, tested, respiratory specimen, performed, absence, 【제목키워드】 detection, SARS-CoV-2 RNA, finding,