ABSTRACT Background: The ability of coronavirus SARS-CoV-2 to spread is one of the determinants of the COVID-19 pandemic status. Until June 2020, global COVID-19 cases surpassed 10 million. Asymptomatic patients, with no respiratory impairment, are believed to be responsible for more than 80% of the transmission. Other viruses have been consistently detected in periodontal tissues. Objective: The aim of this study was to investigate the presence of SARS-CoV-2 in periodontal tissue. Methods: We conducted video-endoscope minimally invasive post-mortem biopsy in seven fatal cases of COVID-19, using a regular endoscope video system associated with a smartphone to locate periodontal tissue. We analyzed the samples using RT-PCR, to identify the SARS-CoV-2 RNA and histopathological analysis. Results: The seven studied autopsies with positive laboratory tests for COVID-19 included 57.14% of female patients at the average age of 47.4 (range 8 to 74). In five cases, periodontal tissue was positive for SARS-CoV-2 (RT-PCR). Histopathologic analyses showed morphologic alterations in the keratinocytes of the junctional epithelium, a vacuolization of the cytoplasm and nucleus and nuclear pleomorphism. Conclusion: We presented a biomolecular analysis obtained from minimally invasive autopsies. This is the first study to demonstrate the presence of SARS-CoV-2 in periodontal tissue in COVID-19 positive patients.
【저자키워드】 COVID-19, infection control, RT-PCR, Autopsy, oral manifestation, 【초록키워드】 SARS-CoV-2, COVID-19 pandemic, Transmission, virus, coronavirus SARS-CoV-2, RNA, Spread, age, Other, patients, Analysis, Laboratory test, Autopsies, cytoplasm, determinant, periodontal tissue, tissues, COVID-19 case, alteration, average, respiratory impairment, nucleus, positive, COVID-19 positive patients, post-mortem biopsy, FIVE, invasive, nuclear pleomorphism, Seven, vacuolization, responsible, analyzed, identify, conducted, histopathological, female patient, surpassed, the SARS-CoV-2, 【제목키워드】 target, Post-mortem, periodontal, tissue,