Abstract Introduction Human leukocyte antigen (HLA) variability has been demonstrated to be associated with susceptibility/severity of COVID‐19. High‐resolution HLA genotyping to identify alleles associated with severe COVID‐19 in an Indian cohort was performed. Methods Quantitative reverse‐transcription polymerase chain reaction‐confirmed SARS‐CoV‐2‐positive patients with mild/moderate/severe disease ( n = 54) and asymptomatic ( n = 42) were recruited and genotyped for 11‐HLA loci on MiSeq using NGSgo®‐MX11‐3 and analyzed (NGSengine; GenDx). Results A significant difference in alleles between the groups was identified for HLA‐C*04:01:01:01, HLA‐DRB5*01:01:01:02, HLA‐DQA1*03:01:01:01, HLA‐DPB1*04:01:01:41, and HLA‐DPA1*01:03:01:02. Alleles namely, HLA‐C*04:01:01:01 (OR: 5.71; 95% CI: 1.2–27.14; p = .02), HLA‐DRB5*01:01:01:02 (OR: 2.94; 95% CI: 1.1–7.84; p = .03), DQA1*03:01:01:01 (OR: 22.47; 95% CI: 1.28–393.5; p = .03), HLA‐DPB1*04:01:01:41 (OR: 9.44; 95% CI: 0.5–175.81; p = .13), and HLA‐DPA1*01:03:01:02 (OR: 8.27; 95% CI: 2.26–30.21; p = .001) were associated with severe COVID‐19. Conclusion Genotyping for these alleles will enable identification of individuals at risk of severe disease and stratification for preferential vaccination.
【저자키워드】 COVID‐19, vaccination, SARS‐CoV‐2, severe Covid‐19, HLA genotyping, 【초록키워드】 Stratification, human leukocyte antigen, risk, COVID‐19, Cohort, Asymptomatic, Patient, HLA, group, disease, genotyping, severe disease, MiSeq, significant difference, individual, Variability, allele, loci, polymerase chain, Result, analyzed, identify, recruited, was performed, demonstrated, genotyped, 【제목키워드】 India, HLA, genotyping, allele, identify,