Summary We developed an intranasal vaccine against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using the replication-incompetent human parainfluenza virus type 2 (hPIV2) vector BC-PIV, which can deliver ectopic gene as stable RNA and ectopic protein on the envelope. BC-PIV expressing the full-length prefusion-stabilized spike gene (K986P/V987P) of SARS-CoV-2, S-2PM, possessed a corona-like viral envelope. Intranasal vaccination of mice with BC-PIV/S-2PM induced high levels of neutralizing immunoglobulin G (IgG) and mucosal IgA antibodies against the spike protein. Although BC-PIV showed hemagglutinating activity, BC-PIV/S-2PM lacked such activity, in accordance with the presence of the massive spike protein on the viral surface. Furthermore, single-dose intranasal vaccination of hamsters with BC-PIV/S-2PM completely protected the lungs from SARS-CoV-2 at 11-week post-immunization, and boost vaccination two weeks before the challenge conferred virtually complete protection of the nasal turbinates against SARS-CoV-2. Thus, this chimeric hPIV2/spike intranasal vaccine is a promising vaccine candidate for SARS-CoV-2 to curtail virus transmission. Graphical abstract Highlights • Non-replicating viral vector against spike induces mucosal immunity to block infection • The viral vector carries spike protein on its envelope with corona-like structure • One-shot nasal vaccination of hamsters completely protects lungs against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) • Two-shot nasal vaccination of hamsters nearly completely protects the upper airway Infection control in health technology; Virology
【저자키워드】 Virology, Infection control in health technology, 【초록키워드】 Viral vector, SARS-CoV-2, IgG, Vaccine, coronavirus, vaccination, Immunity, Infection, lung, nasal, virus transmission, Spike protein, airway, RNA, Protein, Health, Immunoglobulin, spike gene, mice, vaccine candidate, Neutralizing, hamster, intranasal, boost, mucosal, chimeric, nasal turbinate, human parainfluenza virus, acute respiratory syndrome, Abstract, viral surface, full-length, viral envelope, Complete, PROTECT, the spike protein, induce, expressing, IgA antibody, curtail, ectopic, 【제목키워드】 SARS-CoV-2, Vaccine, nasal, human parainfluenza virus, PROTECT, lower airway,