Summary Glycosylation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein mediates viral entry and immune escape. While glycan site is determined by viral genetic code, glycosylation is completely dependent on host cell post-translational modification. Here, by producing SARS-CoV-2 virions from various host cell lines, viruses of different origins with diverse spike protein glycan patterns were revealed. Binding affinities to C-type lectin receptors (CLRs) DC&L-SIGN differed in the different glycan pattern virions. Although none of the CLRs supported viral productive infection, viral trans &cis-infection mediated by the CLRs were substantially changed among the different virions. Specifically, trans &cis-infection of virions with a high-mannose structure (Man 5 GlcNAc 2 ) at the N1098 glycan site of the spike postfusion trimer were markedly enhanced. Considering L-SIGN co-expression with ACE2 on respiratory tract cells, our work underlines viral epigenetic glycosylation in authentic viral infection and highlights the attachment co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention. Graphical abstract Highlights • DC&L-SIGN are SARS-CoV-2 attachment co-receptor • Viral spike (S) glycoprotein undergoes epigenetic modification during infection • Epigenetic glycosylation affects viral in- cis &- trans infections through DC&L-SIGN • High-mannose glycan at 1098 site of postfusion S trimer is vital for viral infection Immunity; Virology; Cell biology; Cell; Glycomics
【저자키워드】 Virology, Immunity, Cell Biology, glycomics, Cell, 【초록키워드】 SARS-CoV-2, viral infection, ACE2, coronavirus, glycosylation, SARS-COV-2 infection, spike glycoprotein, Infection, viral entry, Spike protein, Immune escape, glycoprotein, receptor, Epigenetic, affinity, CLR, L-SIGN, genetic code, host cell, post-translational modification, acute respiratory syndrome, Abstract, CLRs, trimer, respiratory tract cells, virion, Modification, virions, GlcNAc, while, Affect, postfusion, co-expression, S trimer, highlight, virus, supported, dependent on, changed, producing, high-mannose, Man, SARS-CoV-2 virion, 【제목키워드】 SARS-CoV-2, viral infection, glycosylation, receptor,