Abstract Toll-like receptor (TLR) can trigger an immune response against virus including SARS-CoV-2. TLR expression/distribution is varying in mesenchymal stromal cells (MSCs) depending on their culture environments. Here, to explore the effect of periodic thermomechanical cues on TLRs, thermally controlled shape-memory polymer sheets with programmable actuation capacity were created. The proportion of MSCs expressing SARS-CoV-2-associated TLRs was increased upon stimulation. The TLR4/7 colocalization was promoted and retained in the endoplasmic reticula. The TLR redistribution was driven by myosin-mediated F-actin assembly. These results highlight the potential of boosting the immunity for combating COVID-19 via thermomechanical preconditioning of MSCs. Graphic abstract Periodic thermal and synchronous mechanical stimuli provided by polymer sheet actuators selectively promoted the expression of SARS-CoV-2-associated TLRs 4 and 7 in adipose-derived MSCs and recruited TLR4 to Endoplasmic reticulum region where TLR7 was located via controlling myosin-mediated F-actin cytoskeleton assembly.
【저자키워드】 COVID-19, Antiviral, biomaterial, Actuation, Shape memory, 【초록키워드】 SARS-CoV-2, immune response, Immunity, TLR4, TLR7, MSCs, TLRs, virus, Polymer, Culture, receptor, synchronous, expression, MSC, stromal cell, Abstract, F-actin, TLR, highlight, combating, proportion, recruited, provided, expressing, retained, driven by, promoted, Periodic, was increased, 【제목키워드】 Toll-like receptor, distribution, expression, stromal cell, modulation, Periodic,