Efficient and effective viral detection methodologies are a critical piece in the global response to COVID-19, with PCR-based nasopharyngeal and oropharyngeal swab testing serving as the current gold standard. With over 100 million confirmed cases globally, the supply chains supporting these PCR testing efforts are under a tremendous amount of stress, driving the need for innovative and accurate diagnostic solutions. Herein, the utility of a direct-to-PCR method of SARS-CoV-2 detection grounded in mechanical homogenization is examined for reducing resources needed for testing while maintaining a comparable sensitivity to the current gold standard workflow of nasopharyngeal and oropharyngeal swab testing. In a head-to-head comparison of 30 patient samples, this initial clinical validation study of the proposed homogenization-based workflow demonstrated significant agreeability with the current extraction-based method utilized while cutting the total resources needed in half.
【초록키워드】 COVID-19, Stress, diagnostic, supply chains, SARS-CoV-2 detection, sensitivity, PCR testing, Viral, nasopharyngeal, oropharyngeal swab, Patient, gold, Viral detection, methodology, Head-to-head comparison, utility, resource, Critical, efficient, confirmed case, head, gold standard, effort, validation study, driving, effective, initial, homogenization, examined, reducing, demonstrated, comparable, 【제목키워드】 COVID-19, protocol, validation, resource, reducing,