We present CoronaHiT, a platform and throughput flexible method for sequencing SARS-CoV-2 genomes (≤ 96 on MinION or > 96 on Illumina NextSeq) depending on changing requirements experienced during the pandemic. CoronaHiT uses transposase-based library preparation of ARTIC PCR products. Method performance was demonstrated by sequencing 2 plates containing 95 and 59 SARS-CoV-2 genomes on nanopore and Illumina platforms and comparing to the ARTIC LoCost nanopore method. Of the 154 samples sequenced using all 3 methods, ≥ 90% genome coverage was obtained for 64.3% using ARTIC LoCost, 71.4% using CoronaHiT-ONT and 76.6% using CoronaHiT-Illumina, with almost identical clustering on a maximum likelihood tree. This protocol will aid the rapid expansion of SARS-CoV-2 genome sequencing globally. Supplementary Information The online version contains supplementary material available at 10.1186/s13073-021-00839-5.
【저자키워드】 SARS-CoV-2, Nanopore, Sequencing, NGS, Genome, Genetic, Multiplexing, ARTIC, 【초록키워드】 pandemic, protocol, SARS-CoV-2 genome, Clustering, MinION, Illumina, platform, SARS-CoV-2 genome sequencing, supplementary material, SARS-CoV-2 genomes, Illumina platforms, rapid expansion, transposase, genome coverage, PCR products, likelihood, flexible, sequenced, demonstrated, was obtained,