As CRISPR/Cas systems have been refined over time, there has been an effort to apply them to real world problems, such as developing sequence-targeted antiviral therapies. Viruses pose a major threat to humans and new tools are urgently needed to combat these rapidly mutating pathogens. Importantly, a variety of CRISPR systems have the potential to directly cleave DNA and RNA viral genomes, in a targeted and easily-adaptable manner, thus preventing or treating infections. This perspective article highlights recent studies using different Cas effectors against various RNA viruses causing acute infections in humans; a latent virus (HIV-1); a chronic virus (hepatitis B); and viruses infecting livestock and animal species of industrial importance. The outlook and remaining challenges are discussed, particularly in the context of tacking newly emerging viruses, such as SARS-CoV-2.
【저자키워드】 SARS-CoV-2, HIV, Antiviral, CRISPR, hepatitis B, Cas13, RNA virus, Cas9, 【초록키워드】 viruses, Human, virus, infections, Hepatitis, acute infection, viral genomes, Pathogens, antiviral therapies, problems, Perspective, effort, effector, infecting, DNA and RNA, highlight, virus, RNA virus, variety, cleave, 【제목키워드】 application, System,