Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of the core neutralising epitope (COE) of PEDV conjugated to polymeric immunoglobulin G scaffold (PIGS) in glycoengineered Nicotiana be nthamiana plants. The biological activity of COE-PIGS was demonstrated by binding to C1q component of the complement system, as well as the surface of antigen-presenting cells (APCs) in vitro. The recombinant COE-PIGS induced humoral and cellular immune responses specific for PEDV after both systemic and mucosal vaccination. Altogether, the data indicated that PEDV antigen fusion to poly-Fc could be a promising vaccine platform against respiratory PEDV infection.
【저자키워드】 Vaccine, Immunity, plant, mucosal, PEDV, 【초록키워드】 vaccination, Cellular immune response, Infection, complement, in vitro, virus, Antigen, plants, Immunoglobulin, vaccine candidate, vaccine platform, epitope, expression, binding, antigen-presenting cell, humoral, epidemic diarrhea, APCS, PIGS, biological activity, effective, neutralising, tested, indicated, demonstrated, the Coronaviridae, increasingly, C1q, COE, 【제목키워드】 immunogenicity, Immunoglobulin G, oral, systemic, Nicotiana benthamiana,