Outbreak of coronavirus disease 2019 occurred in Wuhan and has rapidly spread to almost all parts of world. GB-1, the herbal formula from Tian Shang Sheng Mu of Chiayi Puzi Peitian Temple, is used for the prophylaxis of SARS-CoV-2 in Taiwan. In this study, we investigated that the effect of GB-1 and the index compounds of GB-1 on the ACE2 and TMPRSS2 expression through in vitro and in vivo study. In our result, GB-1 can inhibit ACE2 and TMPRSS2 protein expression in HepG2 cells, 293T cells, and Caco-2 cells without cytotoxicity. For the mouse model, GB-1 treatment could decrease ACE2 and TMPRSS2 expression levels of the lung and kidney tissue without adverse effects, including nephrotoxicity and hepatotoxicity. In the compositions of GB-1, 0.5–1 mg/ml of Glycyrrhiza uralensis Fisch. ex DC . extract could not inhibit ACE2 mRNA and protein expression in HepG2 cells. In addition, theaflavin-3-gallate could inhibit protein expression of ACE2 and TMPRSS2 without significant cytotoxicity. Our results suggest that GB-1 and theaflavin-3-gallate could act as potential candidates for prophylaxis or treatment of SARS-CoV-2 infection through inhibiting protein expression of ACE2 and TMPRSS2 for the further study.
【저자키워드】 SARS-CoV-2, ACE2, TMPRSS, theaflavin, theaflavin 3-gallate, (+)-catechin 4, TMPRSS2, 【초록키워드】 Treatment, coronavirus disease, SARS-COV-2 infection, lung, cytotoxicity, in vitro, Prophylaxis, adverse effects, Wuhan, in vivo, Taiwan, expression, Glycyrrhiza uralensis, Compound, ACE2 mRNA, TMPRSS2 expression, candidate, protein expression, TMPRSS2 protein, kidney tissue, HepG2 cells, decrease, 293T cells, occurred, spread to, addition, investigated, inhibit, inhibiting, Tian, Caco-2 cell, Temple, TMPRSS2 expression level, 【제목키워드】 Simultaneous, Potential,