ABSTRACT Although frequently reported since the beginning of the pandemic, questions remain regarding the impact of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) interaction with circulating respiratory viruses in coinfected patients. We here investigated dual infections involving early-pandemic SARS-CoV-2 and the Alpha variant and three of the most prevalent respiratory viruses, rhinovirus (RV) and Influenza A and B viruses (IAV and IBV), in reconstituted respiratory airway epithelial cells cultured at air–liquid interface. We found that SARS-CoV-2 replication was impaired by primary, but not secondary, rhino- and influenza virus infection. In contrast, SARS-CoV-2 had no effect on the replication of these seasonal respiratory viruses. Inhibition of SARS-CoV-2 correlated better with immune response triggered by RV, IAV and IBV than the virus entry. Using neutralizing antibody against type I and III interferons, SARS-CoV-2 blockade in dual infections could be partly prevented. Altogether, these data suggested that SARS-CoV-2 interaction with seasonal respiratory viruses would be modulated by interferon induction and could impact SARS-CoV-2 epidemiology when circulation of other respiratory viruses is restored.
【저자키워드】 COVID-19, SARS-CoV-2, variants of concern, interferon, Influenza virus, rhinovirus, Alpha variant, viral co-infections, 【초록키워드】 neutralizing antibody, immune response, pandemic, Infection, interferons, inhibition, virus, coronavirus 2, Replication, respiratory viruses, respiratory virus, virus entry, circulation, respiratory, patients, SARS-CoV-2 replication, Interaction, SARS-CoV-2 epidemiology, These data, type I, circulating, blockade, air–liquid interface, IBV, IAV, prevalent, reported, investigated, question, suggested, correlated, triggered, prevented, had no, restored, other respiratory virus, was impaired, modulated, airway epithelial cell, 【제목키워드】 Influenza, Infection, upper respiratory tract, modulate, replicative,