ABSTRACT Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) has resulted in a pandemic and is continuing to spread rapidly around the globe. No effective vaccine is currently available to prevent COVID-19, and intense efforts are being invested worldwide into vaccine development. In this context, all technology platforms must overcome several challenges resulting from the use of an incompletely characterized new virus. These include finding the right conditions for virus amplification for the development of vaccines based on inactivated or attenuated whole viral particles. Here, we describe a shotgun tandem mass spectrometry workflow, the data produced can be used to guide optimization of the conditions for viral amplification. In parallel, we analysed the changes occurring in the host cell proteome following SARS-CoV-2 infection to glean information on the biological processes modulated by the virus that could be further explored as potential drug targets to deal with the pandemic.
【저자키워드】 COVID-19, SARS-CoV-2, proteomics, Vaccine, mass spectrometry, host response, infection kinetics, viral protein detection, 【초록키워드】 Vaccine development, coronavirus, pandemic, SARS-COV-2 infection, virus, Spread, amplification, drug target, proteome, information, change, platform, inactivated, Tandem mass spectrometry, Viral particles, host cell, effort, new virus, Prevent, effective, viral amplification, produced, resulting, globe, include, analysed, characterized, can be used, condition, overcome, biological processe, modulated, 【제목키워드】 Antigen, Analysis, shotgun, viral particle, SARS-CoV-2-infected cell,