The advent of portable nanopore sequencing devices has enabled DNA and RNA sequencing to be performed in the field or the clinic. However, advances in in situ genomics require parallel development of portable, offline solutions for the computational analysis of sequencing data. Here we introduce Genopo , a mobile toolkit for nanopore sequencing analysis. Genopo compacts popular bioinformatics tools to an Android application, enabling fully portable computation. To demonstrate its utility for in situ genome analysis, we use Genopo to determine the complete genome sequence of the human coronavirus SARS-CoV-2 in nine patient isolates sequenced on a nanopore device, with Genopo executing this workflow in less than 30 min per sample on a range of popular smartphones. We further show how Genopo can be used to profile DNA methylation in a human genome sample, illustrating a flexible, efficient architecture that is suitable to run many popular bioinformatics tools and accommodate small or large genomes. As the first ever smartphone application for nanopore sequencing analysis, Genopo enables the genomics community to harness this cheap, ubiquitous computational resource. Samarakoon et al. present Genopo , a portable toolkit for analysis of nanopore sequencing data on Android smartphone and tablet devices. Genopo enables field-based nanopore analysis using widely accessible technology.
【저자키워드】 Computational platforms and environments, DNA sequencing, 【초록키워드】 Nanopore, Sequencing, Genome, bioinformatics, coronavirus SARS-CoV-2, Community, genomes, DNA methylation, utility, resource, Analysis, genome sequence, Human genome, computation, Tablet, toolkit, sequencing data, computational analysis, Sequencing analysis, Complete, DNA and RNA, flexible, performed, sequenced, nine, can be used, less, determine, patient isolate, 【제목키워드】 Nanopore, toolkit, Sequencing analysis,