Sequence analysis showed heterogeneity among 74 strains and distinct molecular characteristics of highly virulent strains and variants. Porcine epidemic diarrhea virus (PEDV), which emerged in the United States in 2013, has spread throughout North America. Limited availability of PEDV complete genomes worldwide has impeded our understanding of PEDV introduction into the United States. To determine the relationship between the North American strains and global emerging and historic PEDV strains, we sequenced and analyzed complete genomes of 74 strains from North America; the strains clustered into 2 distinct clades. Compared with the initially reported virulent US PEDV strains, 7 (9.7%) strains from 4 states contained insertions and deletions in the spike gene (S INDELs). These S INDEL strains share 99.8%–100% nt identity with each other and 96.2%–96.7% nt identity with the initial US strains. Furthermore, the S INDEL strains form a distinct cluster within North American clade II, sharing 98.6%–100% nt identity overall. In the United States, the S INDEL and original PEDV strains are co-circulating and could have been introduced simultaneously.
【저자키워드】 viruses, Phylogenetic analysis, spike gene, single nucleotide polymorphisms, Porcine epidemic diarrhea virus, PIGS, open reading frame 1, virulent strains, S INDEL strains, complete genome analysis, recombination identification analysis, PEDV variant, US strains, ORF 1, 【초록키워드】 Genome, virus, variants, heterogeneity, Spread, Characteristics, Cluster, Deletion, clade, clades, molecular, Strains, Analysis, strain, epidemic diarrhea, identity, North America, PEDV, North, insertion, virulent, The United States, Complete, initial, analyzed, sequenced, reported, the United State, determine, introduced, Limited, 【제목키워드】 North America, PEDV,