We developed a set of three real-time reverse transcription–polymerase chain reaction (PCR) assays that amplify three different regions of the SARS-associated coronavirus (SARS-CoV), can be run in parallel or in a single tube, and can detect <10 genome equivalents of SARS-CoV. The assays consider all currently available SARS-CoV sequences and are optimized for two prominent real-time PCR platforms.
All Keywords
【저자키워드】 coronavirus, SARS, molecular diagnostics, real-time RT-PCR, 【초록키워드】 coronavirus, SARS-CoV, Genome, detection, Region, PCR, Real-time PCR, assay, sequence, different, detect, amplify,
【저자키워드】 coronavirus, SARS, molecular diagnostics, real-time RT-PCR, 【초록키워드】 coronavirus, SARS-CoV, Genome, detection, Region, PCR, Real-time PCR, assay, sequence, different, detect, amplify,