Since its emergence in Wuhan, China, in December 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected ≈6 million persons worldwide. As SARS-CoV-2 spreads across the planet, we explored the range of human cells that can be infected by this virus. We isolated SARS-CoV-2 from 2 infected patients in Toronto, Canada; determined the genomic sequences; and identified single-nucleotide changes in representative populations of our virus stocks. We also tested a wide range of human immune cells for productive infection with SARS-CoV-2. We confirm that human primary peripheral blood mononuclear cells are not permissive for SARS-CoV-2. As SARS-CoV-2 continues to spread globally, it is essential to monitor single-nucleotide polymorphisms in the virus and to continue to isolate circulating viruses to determine viral genotype and phenotype by using in vitro and in vivo infection models.
【저자키워드】 COVID-19, 2019 novel coronavirus disease, coronavirus disease, severe acute respiratory syndrome coronavirus 2, viruses, respiratory infections, zoonoses, SARS-CoV-2, Replication, Isolation, phylogenetics, immune cells, 【초록키워드】 coronavirus, Infection, polymorphism, in vitro, virus, Population, Peripheral blood, Spread, Genotype, phenotype, in vivo, genomic, Immune cell, mononuclear cell, acute respiratory syndrome, virus stocks, circulating, Toronto, MONITOR, human cell, Wuhan, China, tested, determine, changes in, infected patient, infection with SARS-CoV-2, single-nucleotide, 【제목키워드】 coronavirus 2, Infectivity, Kinetics, respiratory,