A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.
All Keywords
【저자키워드】 coronavirus, SARS, RT-PCR, Real-time PCR, 【초록키워드】 coronavirus, SARS-CoV, Genome, RT-PCR, Region, Culture, Isolation, real-time RT-PCR, application, genomic, specimens, RT-PCR assay, Detection limit, reaction, chain, SARS-CoV infection, primer, diagnosing, probe, different, detect, animal coronavirus,
【저자키워드】 coronavirus, SARS, RT-PCR, Real-time PCR, 【초록키워드】 coronavirus, SARS-CoV, Genome, RT-PCR, Region, Culture, Isolation, real-time RT-PCR, application, genomic, specimens, RT-PCR assay, Detection limit, reaction, chain, SARS-CoV infection, primer, diagnosing, probe, different, detect, animal coronavirus,