Coronavirus NL63 was found in hospitalized children with upper and lower respiratory infections. The human coronavirus NL63 (HCoV-NL63) was first identified in the Netherlands, and its circulation in France has not been investigated. We studied HCoV-NL63 infection in hospitalized children diagnosed with respiratory tract infections. From November 2002 to April 2003, we evaluated 300 respiratory specimens for HCoV-NL63. Of the 300 samples, 28 (9.3%) were positive for HCoV-NL63. The highest prevalence was found in February (18%). The main symptoms were fever (61%), rhinitis (39%), bronchiolitis (39%), digestive problems (33%), otitis (28%), pharyngitis (22%), and conjunctivitis (17%). A fragment of the spike protein gene was sequenced to determine the variety of circulating HCoV-NL63. Phylogenetic analysis indicated that strains with different genetic markers cocirculate in France.
【저자키워드】 Pneumonia, Phylogenetic analysis, Multiplex assay, molecular detection, Bronchiolitis, coronavirus NL63, 【초록키워드】 respiratory infections, coronavirus, Hospitalized, children, Infection, Symptom, Respiratory tract infections, Human coronavirus, Prevalence, Fever, France, NL63, circulation, HCoV-NL63, Analysis, strain, Netherlands, problem, circulating, positive, genetic marker, highest, found, respiratory specimen, different, 2003, sequenced, indicated, investigated, diagnosed, evaluated, determine, variety, the spike protein, digestive,