Co-infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other viruses has been reported. We evaluated cell lines commonly used to isolate viruses and diagnose related diseases for their susceptibility to SARS-CoV-2. Although multiple kidney cell lines from monkeys were susceptible to SARS-CoV-2, we found many cell types derived from humans, dogs, minks, cats, mice, and chicken were not. We analyzed MDCK cells, which are most commonly used for surveillance and study of influenza viruses, and found that they were not susceptible to SARS-CoV-2. The low expression level of the angiotensin converting enzyme 2 receptor and lower receptor affinity to SARS-CoV-2 spike, which could be overcome by overexpression of canine angiotensin converting enzyme 2 in trans, strengthened the cellular barrier to productive infection. Moreover, a D614G mutation in the spike protein did not appear to affect SARS-CoV-2 cell tropism. Our findings should help avert inadvertent propagation of SARS-CoV-2 from diagnostic cell lines.
【저자키워드】 COVID-19, coronavirus disease, viruses, respiratory infections, zoonoses, SARS-CoV-2, ACE2, Influenza, severe acute respiratory syndrome Coronavirus, angiotensin-converting enzyme 2, cell lines, MDCK, spike protein substitution, 【초록키워드】 coronavirus, susceptibility, Infection, diagnostic, kidney, D614G mutation, Surveillance, mice, humans, influenza viruses, canine, receptor, disease, diagnose, SARS-CoV-2 spike, cellular, cell type, acute respiratory syndrome, enzyme, low expression, help, cell line, overexpression, Affect, susceptible, analyzed, virus, reported, evaluated, the spike protein, overcome, MDCK cells, SARS-CoV-2 cell, 【제목키워드】 Other, substrate, isolate, LINE,